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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 4047362, 18 pages
http://dx.doi.org/10.1155/2016/4047362
Research Article

Colonic and Hepatic Modulation by Lipoic Acid and/or N-Acetylcysteine Supplementation in Mild Ulcerative Colitis Induced by Dextran Sodium Sulfate in Rats

1Faculdade de Nutrição (FANUT), Universidade Federal de Alagoas (UFAL), Campus A. C. Simões, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil
2Pós-Graduação em Ciências da Saúde (PPGCS/UFAL), Maceió, AL, Brazil
3Instituto de Química e Biotecnologia (IQB/UFAL), Maceió, AL, Brazil
4Laboratório de Reatividade Cardiovascular (LRC/UFAL), Maceió, AL, Brazil
5Departamento de Fisiologia e Farmacologia (DFF), Centro de Biociências (CB), Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil
6Rede Nordeste de Biotecnologia (RENORBIO), Recife, PE, Brazil

Received 22 June 2016; Revised 3 October 2016; Accepted 10 October 2016

Academic Editor: Karina Reyes-Gordillo

Copyright © 2016 Fabiana Andréa Moura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Lipoic acid (LA) and N-acetylcysteine (NAC) are antioxidant and anti-inflammatory agents that have not yet been tested on mild ulcerative colitis (UC). This study aims to evaluate the action of LA and/or NAC, on oxidative stress and inflammation markers in colonic and hepatic rat tissues with mild UC, induced by dextran sodium sulfate (DSS) (2% w/v). LA and/or NAC (100 mg·kg·day−1, each) were given, once a day, in the diet, in a pretreatment phase (7 days) and during UC induction (5 days). Colitis induction was confirmed by histological and biochemical analyses (high performance liquid chromatography, spectrophotometry, and Multiplex®). A redox imbalance occurred before an immunological disruption in the colon. NAC led to a decrease in hydrogen peroxide (H2O2), malondialdehyde (MDA) levels, and myeloperoxidase activity. In the liver, DSS did not cause damage but treatments with both antioxidants were potentially harmful, with LA increasing MDA and LA + NAC increasing H2O2, tumor necrosis factor alpha, interferon gamma, and transaminases. In summary, NAC exhibited the highest colonic antioxidant and anti-inflammatory activity, while LA + NAC caused hepatic damage.