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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 8296150, 10 pages
http://dx.doi.org/10.1155/2016/8296150
Research Article

Proliferation of Human Primary Myoblasts Is Associated with Altered Energy Metabolism in Dependence on Ageing In Vivo and In Vitro

1Institute of Biomedicine and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia
2Department of Traumatology and Orthopaedics, Tartu University Hospital, L. Puusepa 8, 51014 Tartu, Estonia
3Department of Traumatology and Orthopaedics, University of Tartu, L. Puusepa 8, 51014 Tartu, Estonia
4Institute of Exercise Biology and Physiotherapy, University of Tartu, Ravila 14a, 50411 Tartu, Estonia

Received 24 September 2015; Accepted 8 December 2015

Academic Editor: Rebeca Acín-Pérez

Copyright © 2016 Reedik Pääsuke et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Ageing is associated with suppressed regenerative potential of muscle precursor cells due to decrease of satellite cells and suppressive intramuscular milieu on their activation, associated with ageing-related low-grade inflammation. The aim of the study was to characterize the function of oxidative phosphorylation (OXPHOS), glycolysis, adenylate kinase (AK), and creatine kinase (CK) mediated systems in young and older individuals. Materials and Methods. Myoblasts were cultivated from biopsies taken by transcutaneous conchotomy from vastus lateralis muscle in young (20–29 yrs, ) and older (70–79 yrs, ) subjects. Energy metabolism was assessed in passages 2 to 6 by oxygraphy and enzyme analysis. Results. In myoblasts of young and older subjects the rate of OXPHOS decreased during proliferation from passages 2 to 6. The total activities of CK and AK decreased. Myoblasts of passage 2 cultivated from young muscle showed higher rate of OXPHOS and activities of CK and AK compared to myoblasts from older subjects while hexokinase and pyruvate kinase were not affected by ageing. Conclusions. Proliferation of myoblasts in vitro is associated with downregulation of OXPHOS and energy storage and transfer systems. Ageing in vivo exerts an impact on satellite cells which results in altered metabolic profile in favour of the prevalence of glycolytic pathways over mitochondrial OXPHOS of myoblasts.