The Role of the Exo-Xis Region in Oxidative Stress-Mediated Induction of Shiga Toxin-Converting Prophages

Figure 8

Expression of selected bacteriophage genes in E. coli MG1655 lysogenic with or , either wild-type (blue columns) or Δexo-xis (yellow columns) at 160 min after UV irradiation (50 J/m²), as estimated by reverse transcription quantitative real-time PCR. The values obtained with untreated cells were used as calibrators and were subtracted from the values determined at particular time points; thus, the presented values indicate the induction of expression of tested genes. The presented results are mean values from 3 independent experiments (biological samples), with error bars indicating SD. The additional panel for E. coli () represents the results of Δexo-xis variant with different scale, due to very small values measured. Statistically significant differences (in t-test) are marked as follows: , , and .