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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 9156510, 13 pages
http://dx.doi.org/10.1155/2016/9156510
Research Article

Properties of Flavonoids Isolated from the Bark of Eysenhardtia polystachya and Their Effect on Oxidative Stress in Streptozotocin-Induced Diabetes Mellitus in Mice

1Laboratory of Natural Products Research, School of Chemical Engineering and Extractive Industries, National Polytechnic Institute, Unidad Profesional Adolfo Lopez Mateos, Av. Instituto Politecnico Nacional S/N, 07708 Ciudad de Mexico, Mexico
2Potosine Institute and Scientific and Technological Research (CONACYT), A.C. Dam Road, San Jose 2055, Lomas 4A Section, 78216 San Luis Potosi, SLP, Mexico

Received 10 April 2016; Accepted 8 June 2016

Academic Editor: Renata Szymanska

Copyright © 2016 Rosa Martha Perez-Gutierrez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Six new flavonoids 2′,4′-dihydroxychalcone-6′-O-β-D-glucopyranoside (1), α,3,2′,4′-tetrahydroxy-4-methoxy-dihydrochalcone-3′-C-β-glucopyranosy-6′-O-β-D-glucopyranoside (2), 7-hydroxy-5,8′-dimethoxy-6′α-L-rhamnopyranosyl-8-(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (3), 6′7-dihydroxy-5,8-dimethoxy-8(3-phenyl-trans-acryloyl)-1-benzopyran-2-one (4), 9-hydroxy-3,8-dimethoxy-4-prenylpterocarpan (5), and α,4,4′-trihydroxydihydrochalcone-2′-O-β-D-glucopyranoside (6) were isolated from bark of Eysenhardtia polystachya. Antidiabetic activity of compounds 15 in terms of their cellular antioxidant and free radical scavenging and also in streptozotocin- (STZ-) induced diabetic mice was evaluated on liver transaminases, lipid peroxidation, total bilirubin, total protein, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (CSH-Px), and glutathione reductase (GSH). Results indicated that 15 scavenged 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl (), nitric oxide radicals (), superoxide anion radical (), radical cation (), and hydrogen peroxide (H2O2) radical, and protection against H2O2 induced BSA damage was also observed. Furthermore, 15 showed ability to decrease the oxidative stress in H9c2 cell. Diabetic mice present high levels of lipid peroxide, total protein, SGPT, SGOT, ALP, and TB. However, treatment of STZ-induced diabetes in mice with 15 reduced levels of these enzymes leading to protector effect of liver. In addition, with treatment with 15, increases in radical scavenging enzymes of CSH-Px, SOD, GSH, and CAT have also been observed in diabetic mice. The antioxidant properties of compounds 15 are a promising strategy for ameliorating therapeutic effects by avoiding disorders in the normal redox reactions in healthy cells which consequently could alleviate complications of diabetes.