Table of Contents Author Guidelines Submit a Manuscript
Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 1401452, 12 pages
Research Article

Associations between Specific Redox Biomarkers and Age in a Large European Cohort: The MARK-AGE Project

1Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke (DIfE), 14558 Nuthetal, Germany
2NutriAct-Competence Cluster Nutrition Research Berlin-Potsdam, 14458 Nuthetal, Germany
3Institute of Biological Chemistry and Nutrition, University of Hohenheim, 70599 Stuttgart, Germany
4URBC-NARILIS, University of Namur, 5000 Namur, Belgium
5National Institute of Public Health and the Environment (RIVM), 3720 BA Bilthoven, Netherlands
6Institute of Biological Research and Biotechnology, National Hellenic Research Foundation (NHRF), 11635 Athens, Greece
7Department of Experimental Pathology, University of Bologna, 40126 Bologna, Italy
8Nencki Institute of Experimental Biology, Polish Academy of Sciences, 02-093 Warsaw, Poland
9School of Medicine, University of Tampere, 33014 Tampere, Finland
10Department of Applied Nutritional Science/Dietetics, Institute of Nutritional Medicine, University of Hohenheim, 70599 Stuttgart, Germany
11Molecular Toxicology, Department of Biology, University of Konstanz, 78457 Konstanz, Germany
12German Center for Diabetes Research (DZD), 85764 Munich-Neuherberg, Germany
13German Center for Cardiovascular Research (DZHK), 13357 Berlin, Germany

Correspondence should be addressed to Daniela Weber; ed.efid@rebew.aleinad

Received 24 February 2017; Revised 29 May 2017; Accepted 15 June 2017; Published 19 July 2017

Academic Editor: Daniela Giustarini

Copyright © 2017 Daniela Weber et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Oxidative stress and antioxidants play a role in age-related diseases and in the aging process. We here present data on protein carbonyls, 3-nitrotyrosine, malondialdehyde, and cellular and plasma antioxidants (glutathione, cysteine, ascorbic acid, uric acid, α-tocopherol, and lycopene) and their relation with age in the European multicenter study MARK-AGE. To avoid confounding, only data from countries which recruited subjects from all three study groups (five of eight centers) and only participants aged ≥55 years were selected resulting in data from 1559 participants. These included subjects from (1) the general population, (2) members from long-living families, and (3) their spouses. In addition, 683 middle-aged reference participants (35–54 years) served as a control. After adjustment for age, BMI, smoking status, gender, and country, there were differences in protein carbonyls, malondialdehyde, 3-nitrotyrosine, α-tocopherol, cysteine, and glutathione between the 3 study groups. Protein carbonyls and 3-nitrotyrosine as well as cysteine, uric acid, and lycopene were identified as independent biomarkers with the highest correlation with age. Interestingly, from all antioxidants measured, only lycopene was lower in all aged groups and from the oxidative stress biomarkers, only 3-nitrotyrosine was increased in the descendants from long-living families compared to the middle-aged control group. We conclude that both lifestyle and genetics may be important contributors to redox biomarkers in an aging population.