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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 2709053, 13 pages
https://doi.org/10.1155/2017/2709053
Research Article

MicroRNA-93 Regulates Hypoxia-Induced Autophagy by Targeting ULK1

1Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 511436, China
2Anti-Stress and Health Research Center, College of Pharmacy, Jinan University, Guangzhou 510632, China
3Department of Anesthesiology, Guangdong Medical University, Zhanjiang 524001, China
4Department of Spine Surgery, Shanghai East Hospital, Tongji University School of Medicine, Tongji University, Shanghai 200120, China
5Institute of Neurology, Guangdong Key Laboratory of Age-Related Cardiac-Cerebral Vascular Disease, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong 524001, China
6Department of Obstetrics and Gynecology, Beijing Hospital, National Center of Gerontology, Beijing 100730, China
7Department of Cardiology, Guangdong Cardiovascular Institute, Guangdong Provincial Key Laboratory of Coronary Heart Disease Prevention, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China
8Department of Neurology, Gongli Hospital, Pudong New Area, Shanghai 200135, China

Correspondence should be addressed to Rongrong He; moc.361@66ehgnorgnor, Liangqing Zhang; moc.361@0791qlgnahz, and Du Feng; moc.liamxof@ud_gnef

Received 30 January 2017; Revised 13 July 2017; Accepted 8 August 2017; Published 3 October 2017

Academic Editor: Savita Khanna

Copyright © 2017 Wen Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1. Transfection efficiency detection of MEFs and CHO cells. A. GFP vectors were transfected into MEFs and CHO cells for 24 h. Cell images were captured with a EVOS FL Auto Cell Imaging System (Thermo Fisher Scientific). GFP-positive transfected cells (green). The nuclei were stained with DAPI (blue). B. Negative control FAM (FAM-siRNA NC) was transfected into MEFs and CHO cells for 24 h. Cell images were captured with a EVOS FL Auto Cell Imaging System (Thermo Fisher Scientific). FAM-siRNA NC (green), DAPI (blue).

  1. Supplementary material