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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 4064628, 17 pages
Research Article

Increasing the Fungicidal Action of Amphotericin B by Inhibiting the Nitric Oxide-Dependent Tolerance Pathway

1Centre of Microbial and Plant Genetics, KU Leuven, 3001 Heverlee, Belgium
2BIOSYST-MEBIOS, KU Leuven, 3001 Heverlee, Belgium
3VIB Department of Plant Systems Biology, 9052 Ghent, Belgium
4Department of Laboratory Medicine, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium
5Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal
6ICVS/3B’s-PT Government Associate Laboratory, Braga/Guimarães, Portugal

Correspondence should be addressed to Bruno P. A. Cammue; eb.nevueluk.wib@eummac.onurb

Received 18 May 2017; Accepted 2 August 2017; Published 10 October 2017

Academic Editor: Reiko Matsui

Copyright © 2017 Kim Vriens et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

S1 Fig. Pearson Product-Moment Correlation analysis suggests a good linear correlation between results obtained in bulk and on the DMF platform. S2 Fig. Kinetics of nitric oxide radical production in yeast cells subjected to AmB. S3 Fig. Schematic overview of the DMF setup. S4 Fig. L-NAME does not significantly alter the killing potential of peroxide in S. cerevisiae. S5 Fig. Time lapse experiments via bulk analysis in S. cerevisiae corroborate the results of the single cell analysis via the DMF platform. S6 Fig. L-NAME acts fungistatic on S. cerevisiae.

  1. Supplementary material