Role of Protein Kinase C and Nox2-Derived Reactive Oxygen Species Formation in the Activation and Maturation of Dendritic Cells by Phorbol Ester and Lipopolysaccharide

Figure 5

Analysis of the effects of genetic knock out of the subunit of the phagocytic NADPH oxidase on ROS formation, maturation, and T cell stimulatory capacity of PDBu- or LPS-activated BM-DCs. BM-DCs of control mice (C57BL/6) and mice were stimulated with LPS (1.5 μg/ml) or PDBu (10 μM). ROS formation was measured after 15 and 120 min in (a) or (b) expressing CD11c⁺ cells. (c) Analysis of maturation represented by high expression of MHCII and CD86 24 h after stimulation. (d) Analysis of T cell stimulatory capacity on day 4. Data are mean ± SEM of (a) 3, (b) 3, (c) 3, and (d) 3 experiments. versus C57BL/6 PDBu at 15 min.

(a)

(b)

(c)

(d)