SIRT1 Activation by Resveratrol Alleviates Cardiac Dysfunction via Mitochondrial Regulation in Diabetic Cardiomyopathy Mice

Figure 2

Cardiac-specific SIRT1 knockout (SIRT1^KO) mice displayed symptoms of DCM. (a) Mouse tail tissue PCR showed different genotypes during the process of crossbreeding. (b) There was almost no SIRT1 mRNA in SIRT1^KO mouse cardiac tissue ( in Heter group, in SIRT1^KO group). (c) SIRT1 protein was also barely expressed in SIRT1^KO mice myocardium ( in SIRT1^KO group). (d) Increased heart weight/tibia length ratio in SIRT1^KO mouse myocardium and ventricle/heart weight in SIRT1^KO mouse myocardium (). (e) The mRNA expressions of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were significantly increased in SIRT1^KO mice (). (f) SIRT1^KO mouse hearts displayed structural changes and fibrogenesis in the myocardium. (g) Cardiac function was impaired in SIRT1^KO mice as compared with WT and Heter mice () (Figure 1(g)). versus WT. WT: wild type; Heter: heterozygous.

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