miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1

Figure 5

MiR-382 abundance and HSPD1 in patients with IgA nephropathy (IgAN). (a) Representative images of miR-382 expression in renal biopsy specimens from IgA nephropathy (IgAN) patients, obtained by in situ hybridization (ISH). (b) Immunohistochemical (IHC) analysis of HSPD1 abundance in renal biopsy specimens. (c) Histogram represented the TaqMan qPCR analysis of miR-382 abundance. Total RNA from formalin-fixed and paraffin-embedded tissue was extracted by using RecoverALL total nucleic acid isolation kit (Ambion, Austin, Texas, USA). (d) Histogram represented the relative protein expression of HSPD1 of renal biopsy specimens from IgAN patients. The positively stained area of HSPD1 protein was quantified as described in Figure 1. TIF indicates tubulointerstitial fibrosis. , ^# compared with IgAN without TIF (IHC, ; ISH, ).

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