miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1

Figure 6

Expression of EMT markers (E-cadherin, α-SMA, and Vimentin) and oxidative stress-related markers (Trx, 3-NT) in patients with IgAN. (a) Representative images of protein expression of E-cadherin, α-SMA, Vimentin, thioredoxin (Trx), and 3-nitrotyrosine (3-NT) in renal biopsy specimens from IgA nephropathy (IgAN) patients. Immunohistochemical analysis was performed between patients with and without tubulointerstitial fibrosis (TIF). Histograms (b–e) represented the relative mRNA expression of E-cadherin, α-SMA, Vimentin, and Trx. (f) Histogram represents the relative protein abundance of 3-NT. The positively stained area of 3-NT protein was quantified as described in Figure 1. , ^# compared with IgAN without TIF (IHC, ; qPCR, ).

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