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Oxidative Medicine and Cellular Longevity
Volume 2017, Article ID 4708516, 16 pages
https://doi.org/10.1155/2017/4708516
Research Article

miR-382 Contributes to Renal Tubulointerstitial Fibrosis by Downregulating HSPD1

1Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China
2Shanghai Institute of Kidney and Dialysis, Shanghai, China
3Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China
4Department of Hematology, Zhongshan Hospital, Fudan University, Shanghai, China
5Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA

Correspondence should be addressed to Mingyu Liang; ude.wcm@gnailm and Xiaoqiang Ding; nc.hs.latipsoh-sz@gnaiqoaix.gnid

Received 2 February 2017; Revised 30 March 2017; Accepted 5 April 2017; Published 7 June 2017

Academic Editor: Jaideep Banerjee

Copyright © 2017 Yi Fang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Figure 1: Expression of α-SMA in obstructed murine kidneys. (A) Immunohistochemical analysis for α-SMA abundance in kidney specimens from mice. Treatment with 10mg/kg anti-miR-382 suppressed the expression of α-SMA in obstructed kidneys but not with the dosage of 20mg/kg. (B) Relative mRNA expression of α-SMA in obstructed murine kidneys. (C) Quantification of α-SMA staining. Values labeled with (*) are compared with the sham group, P<0.05. Values labeled with (#) are compared with 10 mg/kg anti-382 group, P<0.05. “UUO” indicates unilateral ureteral obstruction. “NC” indicates negative control. (n=4). Supplemental Figure 2: Expression of Vimentin in obstructed murine kidneys. (A) Immunohistochemical analysis for Vimentin abundance in kidney specimens from mice. Treatment with 10mg/kg dosage of anti-miR-382 suppressed the expression of Vimentin in UUO mice, but not with the dosage of 20mg/kg. (B) Relative mRNA expression of Vimentin. (C) Quantification of Vimentin staining. Values labeled with (*) are compared with the sham group, P<0.05. Values labeled with (#) are compared with 10 mg/kg anti-382 group, P<0.05. “UUO” indicates unilateral ureteral obstruction. “NC” indicates negative control. (n=4)

  1. Supplementary Material