β-Naphthoflavone-Induced Mitochondrial Respiratory Damage in Cyp1 Knockout Mouse and in Cell Culture Systems: Attenuation by Resveratrol Treatment

Figure 5

Effects of BNF and resveratrol treatment on CYP1A1/1A2 mRNA and protein expression levels. Immunoblot analysis of hepatic mitochondrial and microsome proteins from wild-type control, BNF-, and resveratrol-treated mice (each) following seven days of treatment. Proteins (50 μg each) were resolved by SDS-PAGE on a 12% gel and subjected to immunoblot analysis with anti-CYP1A1/1A2 antibodies. Representative blot from one treated and untreated liver each has been presented. As described in Figure 4, all the three blocks of membranes were derived from the same blot. The blots were also probed with an antibody to the mitochondrion-specific marker voltage-dependent anion channel (VDAC) as loading controls (a). CYP1A1 (b) and CYP1A2 (c) mRNA levels were measured by real-time PCR in Wt control, BNF-, and resveratrol-treated mice as described in Materials and Methods. Results represent mean ± SEM from 4 independent experiments. denotes .

(a)

(b)

(c)