Novel Therapeutic Effects of Leonurine On Ischemic Stroke: New Mechanisms of BBB Integrity

Figure 2

SCM-198 protected against TJ degradation induced by cerebral ischemic reperfusion. (a, d) Western blots of peri-ischemic region and striatum tissues in the tMCAO model showed increased the loss of occludin and ZO-1 levels, but reversed by SCM-198 treatment. GAPDH was used as the loading control. (b-c, e-f) The quantitative analysis occludin and ZO-1 levels were calculated and expressed relative to control. F (3, 12) = 18.65, , Figure 2(b); F (3, 22) = 38.22, , Figure 2(c); F (3, 8) = 8.228, , Figure 2(e); F (3, 16) = 17.23, , Figure 2(f). (g–j) Posttreatment with SCM-198 at 2 h and 0.5 h after reperfusion notably improved occludin and ZO-1 mRNA expression. SCM-198 revealed significantly protection. F (3, 6) = 25.11, , Figure 2(g); F (3, 8) = 7.631, , Figure 2(h); F (3, 8) = 10.04, , Figure 2(i); F (3, 8) = 9.389, , Figure 2(j). Values are expressed as mean ± SEM. ^# versus the control group, versus the tMCAO group, .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)