Novel Therapeutic Effects of Leonurine On Ischemic Stroke: New Mechanisms of BBB Integrity

Figure 5

SCM-198 reduced the degradation of TJs induced by OGD/R. (a) FITC-dextran was used to determine BBB integrity. After reperfusion the leakage of dextran in the plate was remarkably increased. SCM-198 could reduce the leakage, F (4, 10) = 6.671, . (b) Expressions of TJs, occludin, claudin-5, and ZO-1 were evaluated by western blot 4 h after reperfusion. OGD/R induced the obvious loss of TJs, and SCM-198 reversed this loss. (c–e) The results were quantified and expressed relative to control, F (4, 10) = 133.1, , Figure 5(c); F (4, 14) = 26.32, , Figure 5(d); F (4, 14) = 22.99, , Figure 5(e). (f–h) The expressions of mRNA levels were detected by real time RT-PCR. SCM-198 could reduce the degradation of TJs at the level of mRNA, F (4, 10) = 7.764, , Figure 5(f); F (4, 15) = 5.983, , Figure 5(g); F (4, 21) = 12.70, , Figure 5(h). (i) Immunofluorescence analysis of occludin and ZO-1, and rhodamine-conjugated phalloidin for stress fibre. Scale bar = 20 μm. The expressions of occludin and ZO-1 were significantly reduced by OGD/R while SCM-198 prevented their reduction. SCM-198 also decreased the formation of stress fibre. Values are expressed as mean ± SEM. ^# versus the control group, versus the OGD group, .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)