Novel Therapeutic Effects of Leonurine On Ischemic Stroke: New Mechanisms of BBB Integrity

Figure 6

SCM-198 mediated the expression of MMP-9, NOX4, and HDAC4 in vitro. (a–d) Western blot images and quantitative analysis of MMP-9, NOX4, and HDAC4 were shown, F (4, 12) = 20.46, , Figure 6(b); F (4, 19) = 20.6, , Figure 6(c); F (4, 10) = 34.23, , Figure 6(d). (e–g) The mRNA levels of HDAC4, NOX4, and MMP-9 were estimated by real-time RT-PCR, F (4, 10) = 9.474, , Figure 6(e); F (4, 10) = 7.831, , Figure 6(f); F (4, 10) = 39.81, , Figure 6(g). (h) The HDAC4 inhibitor Taq (0.2 μM) or vehicle was applied 1 h before until the end of the experiment. Taq exacerbated the production of ROS, while SCM-198, 1 and 10 μM, could still reduce the ROS formation, F (4, 14) = 9.319, , scale bar = 2 μm. Values are expressed as mean ± SEM. ^# versus the control group, versus the OGD group, .

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