RTA-408 Protects Kidney from Ischemia-Reperfusion Injury in Mice via Activating Nrf2 and Downstream GSH Biosynthesis Gene

Figure 6

GSH biosynthesis and metabolic enzyme activity were induced by RTA-408. The injured kidney was harvested at 24 h reperfusion, then homogenated, and processed for glutathione-related enzyme activity assay. Glutamyl-cysteine ligase (GCL), glutathione peroxidase (GPx), and glutathione S-transferase (GST) enzyme activities (Figures 6(a)–6(c)) and glutathione reductase activation coefficient (GRAC) value (Figure 6(d)) were measured in the supernatant of renal homogenate following the manufacturer’s instructions. Values were expressed as mean ± SD. Significant difference versus the vehicle + I/R group (Figure 6(a), ; Figure 6(b), ; and Figure 6(d), ).

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