Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis

Figure 1

Uptake of AGE-2 and AGE-3 in macrophages. J774.1 cells (10⁶/ml, 100 μl) were plated in 96-well black/clear plates in the culture medium without FBS. After adhesion was allowed for 1 hr, cells were incubated with BSA-AGE-2 or BSA-AGE-3 (20 μg/ml) for 2 hrs. The incorporated BSA-AGE-2 or BSA-AGE-3 was detected by a specific polyclonal antibody made in our lab, and then a secondary antibody (AlexaFluor 488-labeled goat anti-rabbit IgG (H+L)) was added to obtain fluorescence. After fixation, the cells were treated with TBS in the presence or absence of 0.3% Triton X-100. Representative images are shown from four separate experiments. The fluorescence intensity was quantitated and the results are summarized in Figure 1(c). The results shown are means ± SEMs of four separate experiments. versus Triton X-100 (−).

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