Phagocytosis of Advanced Glycation End Products (AGEs) in Macrophages Induces Cell Apoptosis

Figure 4

Effects of endocytosis inhibitors or RAGE inhibitor on AGE-2 and AGE-3 uptake in J774.1 macrophages. The J774.1 macrophages were preincubated with different concentrations of cytochalasin D (a), chlorpromazine (b), and FPS-ZM1 (RAGE inhibitor) (c) at 37°C for 1 hr. Then, the cells were incubated with AGE-2 or AGE-3 (20 μg/ml) at 37°C for 30 min. AGE-2 or AGE-3 was detected as shown in Figure 1. Representative images from three separate experiments are shown.

(a)

(b)

(c)