Hydrogen Sulfide Inhibits Autophagic Neuronal Cell Death by Reducing Oxidative Stress in Spinal Cord Ischemia Reperfusion Injury

Figure 3

Autophagy promotes neuronal cell apoptosis in SCIR injury. (a) Immunofluorescence analysis of TUNEL and LC3 in the spinal cord after I/R treated with or without 3-MA. (b) Immunofluorescence analysis of caspase-3 in the spinal cord after I/R treated with or without 3-MA. (c) Western blot analysis of p62, Atg12-5, LC3-II, and cleaved caspase-3 after I/R injury treated with or without 3-MA. (d) Densitometric analysis of the immunoblot reported in Figure 3(c). (e) BBB scores of animals after SCIR treated with or without 3-MA. Images represent six rats with I/R treated with or without 3-MA. Scale bars represent 10 μm. . Data were analyzed using one-way ANOVA in (d) and two-way ANOVA in (e) and represent three independent experiments. SCIR: spinal cord ischemia reperfusion; TUNEL: terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling; LC3: microtubule-associated protein 1 light chain 3; I/R: ischemia reperfusion; 3-MA: 3-methyladenine; BBB: Basso, Beattie, and Bresnahan; ANOVA: analysis of variance.

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