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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 8759764, 9 pages
Research Article

Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells

1Department of Physiology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
2Department of Chemistry, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
3Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
4Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research “Vinca”, University of Belgrade, Belgrade, Serbia

Correspondence should be addressed to Lada Živković

Received 21 September 2016; Revised 31 October 2016; Accepted 11 January 2017; Published 21 February 2017

Academic Editor: Chung-Yen Oliver Chen

Copyright © 2017 Lada Živković et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 μg/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15 min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.