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Oxidative Medicine and Cellular Longevity
Volume 2017 (2017), Article ID 9210862, 11 pages
Research Article

Immune Profile of Obese People and In Vitro Effects of Red Grape Polyphenols on Peripheral Blood Mononuclear Cells

1Department of Basic Medical Sciences, Neuroscience and Sensory Organs, University of Bari, 70124 Bari, Italy
2Fondazione San Raffaele, 72013 Ceglie Messapica, Italy
3Consorzio MEBIC, San Raffaele University, Rome, Italy
4Farmalabor Srl, 76012 Canosa di Puglia, Italy
5Department of Biomedical Sciences and Human Oncology, Section of Clinical Oncology, University of Bari, 70124 Bari, Italy

Correspondence should be addressed to Emilio Jirillo; ti.abinu@ollirij.oilime

Received 15 June 2016; Accepted 7 December 2016; Published 24 January 2017

Academic Editor: Steven McAnulty

Copyright © 2017 Thea Magrone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The in vitro ability of polyphenols, extracted from red grape, to modulate peripheral blood mononuclear cell responses has been evaluated in 20 obese (Ob) people. With regard to cytokine release in response to phorbol myristate acetate (PMA), levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4, IL-10, and IL-17 were higher in the Ob than in healthy (H) subjects. Vice versa, IL-21 concentrations were detected only in H people but they were undetectable in the Ob counterpart. In general terms, levels of IL-1β, IL-6, IL-8, and tumor necrosis factor-α were higher in Ob people when compared to H controls. On the other hand, polyphenols did not modify IFN-γ, IL-4, and IL-17 levels. However, an increase in IL-2 was observed in H individuals, whereas its levels were decreased in the Ob counterpart. Polyphenols significantly increased IL-10 release from H donors, whereas a trend to increase was observed in Ob people. In addition, polyphenols were able to significantly increase levels of H IL-21, while this was not the case in Ob people. Since IL-21 is an inducer of Th17 cells, it is likely that polyphenols may suppress the sources of this cytokine via production of IL-10. Accordingly, polyphenols decreased IL-1β and IL-6 release in comparison to H controls.