miR-128 Is Implicated in Stress Responses by Targeting MAFG in Skeletal Muscle Cells

Figure 4

miR-128 interferes with the DEM-mediated induction of ARE-dependent genes. C2C12 cells were transiently transfected with miR-128 expressing construct or with an equivalent amount of empty plasmid. 36 h after transfections, the cells were treated for 3 h with 200 μM DEM and then harvested. The mRNA levels of GCLC, GSTA-1, HMOX-1, NQO1, x-CT, and Nrf2 genes were determined by RT-qPCR on total RNA after normalization with the c-ABL mRNA level. Data are expressed as relative to the values obtained upon transfection with CMV-neo control in the absence of DEM, which were set equal to 1. Each column in the panels represents the mean ± SD of 3 independent experiments. .