Nonylphenol and Octylphenol Differently Affect Cell Redox Balance by Modulating the Nitric Oxide Signaling

Figure 3

Evaluation of the estrogen signal involvement in the NOS’s expression and eNOS phosphorylation. HepG2 cells were incubated 24 h with E2 (1 μM), OP (10 μM), and NP (20 μM) in the absence and presence of Flv (1 μM), and Western blot analysis has been carried out using antibodies against (a) eNOS, (b) iNOS, (c) P-S1177 eNOS, and (d) P-T495 eNOS; α-tubulin as reference. (a, b) Densitometric values are shown as fold increase versus the proteins expressed by the control cells. (c, d) Densitometric values are shown as the ratio of phosphorylated eNOS (at Ser1177 or Thr495)/total eNOS (data ± SEM, n ≥ 3). values from Figure 3(a) (not displayed in the picture): E2 versus NP: ; OP versus NP: . values were considered statistically significant by ANOVA.

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