Table of Contents Author Guidelines Submit a Manuscript
Oxidative Medicine and Cellular Longevity
Volume 2018, Article ID 8587475, 7 pages
https://doi.org/10.1155/2018/8587475
Research Article

ROS-Induced DNA Damage Associates with Abundance of Mitochondrial DNA in White Blood Cells of the Untreated Schizophrenic Patients

1Research Centre for Medical Genetics (RCMG), Moscow 115478, Russia
2N. A. Alexeev Clinical Psychiatric Hospital №1 of Moscow Healthcare Department, Moscow 115447, Russia
3V. A. Negovsky Research Institute of General Reanimatology, Federal Clinical Research Center of Reanimatology and Rehabilitogy, Moscow 107031, Russia
4Mental Health Research Center, Moscow 115522, Russia

Correspondence should be addressed to S. V. Kostyuk; moc.liamg@kuytsok.tevs

Received 31 July 2017; Revised 1 November 2017; Accepted 10 December 2017; Published 25 February 2018

Academic Editor: Tanea T. Reed

Copyright © 2018 I. V. Chestkov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The aim of this study was (1) to examine the leukocyte mtDNA copy number (CN) in unmedicated (SZ (m−)) and medicated (SZ (m+)) male patients with paranoid schizophrenia (SZ) in comparison with the healthy male controls (HC) and (2) to compare the leukocyte mtDNA CN with the content of an oxidation marker 8-oxodG in lymphocytes of the SZ (m−) patients. Methods. We evaluated leukocyte mtDNA CN of 110 subjects with SZ in comparison with 60 male HC by the method qPCR (ratio mtDNA/nDNA (gene B2M) was detected). SZ patients were divided into two subgroups. The patients of the subgroups SZ (m+) () were treated with standard antipsychotic medications in the hospital. The patients of the subgroup SZ (m−) () were not treated before venous blood was sampled. To evaluate oxidative DNA damage, we quantified the levels of 8-oxodG in lymphocytes (flow cytometry) of SZ (m−) patients () and HC (). Results. The leukocyte mtDNA CN showed no significant difference in SZ (m+) patients and HC. The mtDNA CN in the unmedicated subgroup SZ (m−) was significantly higher than that in the SZ (m+) subgroup or in HC group. The level of 8-oxodG in the subgroup SZ (m−) was significantly higher than that in HC group. Conclusion. The leukocytes of the unmedicated SZ male patients with acute psychosis contain more mtDNA than the leukocytes of the male SZ patients treated with antipsychotic medications or the healthy controls. MtDNA content positively correlates with the level of 8-oxodG in the unmedicated SZ patients.