|
| Experiment | Measurements | | No. of samples | No. of experiments | Figure |
|
| Ctrl | [Ca2+]mt () | | 30 | 6 | 1(e) |
| MCU | [Ca2+]mt () | | 30 | 6 | 1(e) |
| Ctrl | Resting mitochondrial Ca2+ level (474/410 ratio) | | 30 | 6 | 1(f) |
| MCU | Resting mitochondrial Ca2+ level (474/410 ratio) | | 30 | 6 | 1(f) |
| Ctrl | Number of objects/cell | | 30 | 3 | 2(c) |
| MCU | Number of objects/cell | | 30 | 3 | 2(c) |
| Ctrl+glutamate | Number of objects/cell | | 30 | 3 | 2(c) |
| MCU+glutamate | Number of objects/cell | | 30 | 3 | 2(c) |
| Ctrl | Mean volume of objects/cell (μm3) | | 30 | 3 | 2(c) |
| MCU | Mean volume of objects/cell (μm3) | | 30 | 3 | 2(c) |
| Ctrl+glutamate | Mean volume of objects/cell (μm3) | | 30 | 3 | 2(c) |
| MCU+glutamate | Mean volume of objects/cell (μm3) | | 30 | 3 | 2(c) |
| Ctrl | Mean surface of objects/cell (μm2) | | 30 | 3 | 2(c) |
| MCU | Mean surface of objects/cell (μm2) | | 30 | 3 | 2(c) |
| Ctrl+glutamate | Mean surface of objects/cell (μm2) | | 30 | 3 | 2(c) |
| MCU+glutamate | Mean surface of objects/cell (μm2) | | 30 | 3 | 2(c) |
| synGFP_48h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synMCU-GFP_48h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synGFP_72h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synMCU-GFP_72h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synGFP_96h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synMCU-GFP_96h | Apparent-infected neurons (%) | | 60 | 3 | 3(b) |
| synGFP_48h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synMCU-GFP_48h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synGFP_72h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synMCU-GFP_72h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synGFP_96h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synMCU-GFP_96h | TUNEL-positive neurons (%) | | 60 | 3 | 3(d) |
| synGFP | TMRM fluorescence (a.u.) | | 10 | 3 | 4(a) |
| synMCU-GFP | TMRM fluorescence (a.u.) | | 10 | 3 | 4(a) |
| synGFP | TMRM fluorescence decay half-life (sec) | | 10 | 3 | 4(d) |
| synMCU-GFP | TMRM fluorescence decay half-life (sec) | | 10 | 3 | 4(d) |
| synGFP | [Ca2+]cyt 19 after glutamate treatment () | | 30 | 3 | 4(f) |
| synMCU-GFP | [Ca2+]cyt 19after glutamate treatment () | | 30 | 3 | 4(f) |
| synGFP_48h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| synMCU-GFP_48h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| synGFP_72h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| synMCU-GFP_72h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| synGFP_96h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| synMCU-GFP_96h (+GLUT) | TUNEL-positive neurons (%) | | 60 | 3 | 4(h) |
| Ctrl | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| Ctrl+Cav1 block | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| Ctrl+Cav2 block | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| MCU | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| MCU+Cav1 block | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| MCU+Cav2 block | Resting cytosolic Ca2+ level (474/410 ratio) | | 100 | 3 | 4(i) |
| synGFP | TUNEL/GFP (a.u.) | 5 | 12 | 3 | 5(d) |
| synMCU-GFP | TUNEL/GFP (a.u.) | | 12 | 3 | 5(d) |
| synGFP | CD45/GFP (a.u.) | | 10 | 3 | 5(e) |
| synMCU-GFP | CD45/GFP (a.u.) | | 13 | 3 | 5(e) |
| synGFP | GFAP/GFP (a.u.) | | 8 | 3 | 5(f) |
| synMCU-GFP | GFAP/GFP (a.u.) | | 10 | 3 | 5(f) |
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