Hypoxia-Inducible Factor-1α Knockdown Plus Glutamine Supplementation Attenuates the Predominance of Necrosis over Apoptosis by Relieving Cellular Energy Stress in Acute Pancreatitis

Figure 5

HIF-1α knockdown attenuated NF-κB nuclear translocation induced by AP, and this effect was enhanced by a concurrent administration of Gln. (a and b) Representative EMSA blots (a) and quantifications (b) of NF-κB DNA-binding activity in pancreatic tissues harvested from the rats as described in Figure 1(a). Data were presented as (). versus sham, ^{^}versus AP, and ^▽versus HIF-1α siRNA. Abbreviations: AP: acute pancreatitis; EMSA: electrophoretic mobility shift assay; Gln: glutamine; HIF-1α: hypoxia-inducible factor-1α; NF-κB: nuclear factor-kappa B; SD: standard deviation.

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