Sedoheptulose-1,7-bisphospate Accumulation and Metabolic Anomalies in Hepatoma Cells Exposed to Oxidative Stress

Figure 3

Temporal changes in metabolism in G6PD-deficient hepatoma cells. (a) G6PD activities in Sc and Gi cells were measured and are expressed in U/mg of cell lysate. Data are , . (b) Sc and Gi cells were untreated or treated with 0.5 and 5 mM H₂O₂ for 4 and 24 hr, and their viabilities were determined. Data are , . (c) Sc and Gi cells were treated with 5 mM H₂O₂ for 0, 15, 30, 60, 90, and 120 min and collected for metabolomic analysis. Data were analyzed as described in the legend of Figure 1. The OPLS-DA score plot of Sc and Gi cells treated for various times is shown.

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