H₂S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins

Figure 2

H₂S or H₂Se suppresses H₂O₂-induced oxidative stress. (a) Visualization of intracellular ROS using DCFDA-H₂. (b) Intracellular ROS level analysis using DCFDA-H₂ intensity relative to the control, normalized by the protein amount. H9C2 cells were pretreated with 30 μM NaHS or 0.3 μM Na₂Se for 30 minutes, then incubated with 200 μM H₂O₂ for additional 24 hours followed by staining with 10 μM DCFDA-H₂ for 15 minutes at 37°C. relative to the control; ^# compared to H₂O₂. .

(a)

(b)