Research Article

H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins

Figure 2

H2S or H2Se suppresses H2O2-induced oxidative stress. (a) Visualization of intracellular ROS using DCFDA-H2. (b) Intracellular ROS level analysis using DCFDA-H2 intensity relative to the control, normalized by the protein amount. H9C2 cells were pretreated with 30 μM NaHS or 0.3 μM Na2Se for 30 minutes, then incubated with 200 μM H2O2 for additional 24 hours followed by staining with 10 μM DCFDA-H2 for 15 minutes at 37°C. relative to the control; # compared to H2O2. .