H₂S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins

Figure 4

H₂S stimulates SCLY/H₂Se signaling. (a) CSE deficiency reduced SCLY protein expression in mouse heart tissues. The heart tissues were isolated from 12-week-old CSE knockout mice and wild-type littermates for analysis of SCLY protein expression by western blotting. . (b) H₂S induces SCLY protein expression. H9C2 cells were treated with 30 μM NaHS for 24 hours followed by western blotting detection of SCLY protein expression. . . (c) H₂S increases the Se level in media. After the cells were treated with 30 μM NaHS for 24 hours, media were collected for analysis of the Se level by using DAN. . . (d) H₂Se induces the protein expressions of GPx1 and TrxR2. After the cells were incubated with 0.3 μM Na₂Se for 30 minutes, the cells were processed with or without selenide washout and continued to culture for 1-3 days; the protein expressions were detected with western blotting. .

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