Research Article

H2S Protects against Cardiac Cell Hypertrophy through Regulation of Selenoproteins

Figure 5

H2S regulates selenoproteins. H9C2 cells were pretreated with 30 μM NaHS or 0.3 μM Na2Se for 30 minutes, then incubated with 200 μM H2O2 for additional 24 hours followed by real-time PCR analysis of GPx1 mRNA ((a) versus all other groups), TrxR2 mRNA (b), SePP1 mRNA ((c) versus control; # versus all other groups), and eEFSec mRNA ((e) versus control; # versus H2O2). . (d) CSE deficiency reduced SePP1 protein expression in mouse heart tissues. The heart tissues were isolated from 12-week-old CSE knockout mice and wild-type littermates for analysis of SCLY protein expression by western blotting. .