Synthetic Imine Resveratrol Analog 2-Methoxyl-3,6-Dihydroxyl-IRA Ameliorates Colitis by Activating Protective Nrf2 Pathway and Inhibiting NLRP3 Expression
C33 inhibits NLRP3 expression and induces the expression of ARE-driven genes in WT colitis mice. (a) WT and Nrf2-/- mice were given C33 (200 mg/kg i.g.), RSV (200 mg/kg i.g.), or vehicle (10% ethanol) daily. Two days later, while the mice continued daily treatment with RSV or C33, they were also given normal drinking water or water containing 2.5% DSS for 7 days as in Figure 2(a). Soluble extracts from the colon of mice were analyzed by Western immunoblotting with antibodies against Ho-1, Gstm1, AKR1B8, or Nqo1. Upper panel, representative images of Western immunoblots. Each lane shows the results for a sample from a single mouse. Actin was used as a loading control. Lower panel, semiquantitative result of blot. The value for the same protein from WT mice treated with vehicle (control) was set at 1. Values are ().,, compared with vehicle-treated mice with same protein and phenotype. ##, compared with RSV-treated mice with same protein and phenotype. (b) Immunohistochemical analysis of the expression of NLRP3 in the colons from WT mice treated as in Figure 2(a) (scale bars, 100 μm; original magnification, ×200). A: mice on normal drinking water without any treatment. B: mice treated with vehicle and on drinking water containing 2.5% DSS. C: mice treated with RSV and on drinking water containing 2.5% DSS. D: mice treated with C33 and on drinking water containing 2.5% DSS. Images represent results from three separate experiments. (c) Semiquantitative results from (b). The WT mice on normal drinking water without any treatment (control) was set at 1. Values are ,;.