Alterations in Organismal Physiology, Impaired Stress Resistance, and Accelerated Aging in Drosophila Flies Adapted to Multigenerational Proteome Instability
Multigenerational proteotoxic stress triggered the upregulation of proteostatic modules. (a) Relative expression of Rpn11, Rpn10, Rpn6, Prosα7, Prosβ5, Prosβ2, and Prosβ1 proteasomal genes in somatic tissues of young NT, G80, and G80-BTZ flies; gene expression was plotted vs. the respective control (NT flies). (b) Representative immunoblotting analysis of 20S-α and Prosβ5 proteasomal subunit expression levels in somatic tissues of young female and male NT and G80 flies cultured (or not) in medium containing the indicated BTZ concentrations. (c) Relative (%) CT-L and C-L proteasome activities in young (Y) or aged (old; O) (≥80% of their lifespan) female/male flies of the shown groups. (d1) Relative expression of ref(2)P, Atg6, and Atg8a genes in somatic tissues of young NT and G80-BTZ flies; gene expression was plotted vs. the respective control NT flies. (d2) Relative (%) cathepsin B, L activity in somatic tissues of young NT, G80, and G80-BTZ flies. (e) Longevity curves of female (e1) and male (e2) flies of the indicated groups exposed to 200 μΜ of the autophagy inhibitor chloroquine (CQ). The Rp49 gene expression was used in (a), (b) as input reference. Bars, ±SD ();;.
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