Research Article | Open Access
Alterations in Organismal Physiology, Impaired Stress Resistance, and Accelerated Aging in Drosophila Flies Adapted to Multigenerational Proteome Instability
Being an assembly of highly sophisticated protein machines, cells depend heavily on proteostatic modules functionality and on adequate supply of energetic molecules for maintaining proteome stability. Yet, our understanding of the adaptations induced by multigenerational proteotoxic stress is limited. We report here that multigenerational (>80 generations) proteotoxic stress in OregonR flies induced by constant exposure to developmentally nonlethal doses of the proteasome inhibitor bortezomib (BTZ) (G80-BTZ flies) increased proteome instability and redox imbalance, reduced fecundity and body size, and caused neuromuscular defects; it also accelerated aging. G80-BTZ flies were mildly resistant to increased doses of BTZ and showed no age-related loss of proteasome activity; these adaptations correlated with sustained upregulation of proteostatic modules, which however occurred at the cost of minimal responses to increased BTZ doses and increased susceptibility to various types of additional proteotoxic stress, namely, autophagy inhibition or thermal stress. Multigenerational proteome instability and redox imbalance also caused metabolic reprogramming being evidenced by altered mitochondrial biogenesis and suppressed insulin/IGF-like signaling (IIS) in G80-BTZ flies. The toxic effects of multigenerational proteome instability could be partially mitigated by a low-protein diet that extended G80-BTZ flies’ longevity. Overall, persistent proteotoxic stress triggers a highly conserved adaptive metabolic response mediated by the IIS pathway, which reallocates resources from growth and longevity to somatic preservation and stress tolerance. Yet, these trade-off adaptations occur at the cost of accelerated aging and/or reduced tolerance to additional stress, illustrating the limited buffering capacity of survival pathways.
Considering that cellular functionality is ensured by the highly wired action of sophisticated protein machines and that proteome instability (also referred to as proteotoxic stress) causes significant detrimental effects, it is not surprising that proteome homeodynamics (proteostasis) is central for cellular functionality and the overall healthspan of organisms . To ensure proteostasis, cells have developed a network of modules to assist protein folding and counteract proteotoxic stress; this network is referred to as the proteostasis network (PN) . PN ensures proteome quality control at both basal conditions and during conditions of proteome instability by addressing the triage decision of fold, hold, or degrade . Key components of the PN are the protein synthesis and sorting/trafficking machineries, the molecular chaperones, and the two main degradation machineries, namely, the autophagy lysosome (ALP) and the ubiquitin proteasome (UPP) pathways [4, 5]. ALP is mainly involved in the degradation of damaged organelles and protein aggregates and consists of microautophagy, chaperone-mediated autophagy, and macroautophagy . On the other hand, UPP ensures protein synthesis quality control and it degrades normal short-lived ubiquitinated proteins and nonrepairable misfolded or unfolded polypeptides . Additional modules of the PN are considered to be the stress-responsive signaling pathways (e.g., heat or oxidative), including those of forkhead box O (Foxo) and nuclear factor erythroid 2-related factor (Nrf2) transcription factors. Nrf2 is involved in cell protection against xenobiotic or oxidative damage [7, 8], while Foxo regulates autophagic and metabolic responses [9, 10].
The 26S eukaryotic proteasome is a complex protein machine of ~2.5 MDa that comprises a 20S core particle (CP) bound to one or two 19S regulatory particles (RP) . The 20S CP is composed of four stacked heptameric rings (two α-type surrounding two β-type rings) that form a barrel-like structure; the caspase- (C-L), trypsin- (T-L), and chymotrypsin- (CT-L) like peptidase activities are located at the β1, β2, and β5 proteasome subunits, respectively. Ubiquitinated polypeptides are degraded by the 26S proteasome [5, 12], while nonnative (e.g., oxidized) polypeptides are likely degraded by the 20S proteasome via chaperone-mediated targeting . Furthermore, proteasome has been implicated in maintaining mitochondrial functionality  suggesting that proper function of proteostatic modules is also critical for cellular energetics and metabolism.
Yet, despite the tight PN regulation, a narrow boundary between health and disease exists, and subtle changes caused, for instance, by genetic mutations, environmental stress, or certain aspects of lifestyle (e.g., obesity) can trigger proteome instability. This process can then gradually overwhelm the PN leading to stress spreading within the same tissue and even across distinct organs by cell nonautonomous mechanisms . Beyond these events, aging is undoubtedly the major risk factor for virtually all protein instability-related diseases. This correlation largely relates to reduced functionality of antistress responses and proteostatic modules during aging [15, 16]. Consistently, proteasomal dysfunction has been correlated with deregulation of the proteostasis network possibly underlying the early offset of aging phenotypes and aging-related diseases [5, 16].
Interestingly, aberrant activation of proteostatic modules marks the onset of carcinogenesis ; it is speculated that increased UPP activity during carcinogenesis is associated with tumor cell adaptation to elevated proteotoxic stress [1, 17]. Consistently, therapeutic targeting of the proteasome is currently used for the treatment of hematological malignancies and remains a challenge for the cure of solid tumors [18, 19]. UPP inhibitors which have demonstrated clinical efficacy include bortezomib (BTZ)  and carfilzomib . BTZ is a slowly reversible inhibitor that binds the catalytic site of the 26S proteasome enabling inhibition of the CT-L and, to a lesser extent, of C-L and T-L activities [19, 21, 22]. Nevertheless, the development of severe adverse effects linked to the usage of proteasome inhibitors, such as peripheral neuropathies and/or cardiovascular diseases, along with inherent or acquired drug resistance remain a significant clinical problem [19, 23]. Acquired resistance to proteasome inhibition has been correlated in cellular models with UPP upregulation and/or mutations of the PSMB5 gene; yet, no mutations have been found in the PSMB5 gene in myeloma patients being refractory to or relapsed from BTZ therapy [24, 25].
By using Drosophila flies as a model organism to study cellular proteostasis in the young organism, during aging and in age-related diseases, we recently found that proteasome functionality is sex-, tissue-, and age-dependent . Here, we report that multigenerational proteotoxic stress in OregonR flies induced by exposure to developmentally nonlethal doses of BTZ increased proteome instability and redox imbalance, reduced fecundity and body size, and caused neuromuscular defects; it also accelerated aging. Furthermore, multigenerational proteome instability caused metabolic reprogramming and increased susceptibility to various types of additional proteotoxic stress, namely, autophagy inhibition or thermal stress.
2. Materials and Methods
2.1. Fly Stocks and Treatment with BTZ
G80-BTZ flies were established by continuous treatment of OregonR flies with 0.5 μΜ of the proteasome inhibitor bortezomib (BTZ) (PS-341) for more than 80 generations (>4 years). Flies were maintained at 23-24°C and 60% relative humidity on a 12 h light : 12 h dark cycle as described before  and were cultured in standard (SM), high protein (4x of the regular protein intake) (HPM), low protein (1/2 of the regular protein intake) (LPM), or low calories (CRM) medium (1x SM diluted 1x with boiled tap water).
2.2. Measurement of Growth and Developmental Effects: Climbing and Longevity Assays
For the effects of multigenerational proteotoxic stress on the developmental processes, five young female flies were placed in Petri dishes containing 1.5% agar dissolved in sour cherry juice and allowed to lay eggs for 24 h; embryos in each dish were then counted and collected using PBS. Forty embryos from each population were transferred in fresh standard medium and monitored for 14 days. Cultures were photographed daily to record growth rates and at the end of the assay, adult flies were counted and each developmental stage (larvae, early/late stage pupae, or adult flies) was captured using a stereomicroscope to probe possible defects during development and alternations in morphology, respectively.
Body size was examined by weighting young flies and by measuring the wings’ perimeter. Twenty young female flies from each population were collected and allowed to lay eggs for 24 h; then, adult flies were removed, and embryos allowed to develop to adulthood. Middle-aged flies were collected in groups of ten individuals and starved for 1 h followed by weighting each group of flies to 0.01 mg accuracy with an analytical balance. Removed wings from middle-aged flies were placed on observation plates and captured using a digital camera adapted to a stereomicroscope, and measurement of the perimeter was performed using Image J (National Institutes of Health, USA).
Longevity and climbing performance were assayed as previously described . For survival curves and statistical analysis, the Kaplan-Meier procedure and log-rank (Mantel-Cox) test were used; significance was accepted at . Statistical analyses for all presented longevity experiments are reported in Table S1.
2.3. Heat and Bang Sensitivity Assays
Heat and bang assays were performed as described before  with minor modifications. Briefly, two groups of fifteen sex-sorted young flies were collected and transferred separately in vials containing fresh medium. For the heat sensitivity assay, flies were incubated (the next day) for 10 min at 40°C in empty vials, whereas for the bang assay (it mostly reveals neuronal defects ), flies were placed in empty vials and were vortexed for 20 sec at room temperature (RT); in both assays, treated flies were allowed to recover at RT. Assays were videotaped, and the time of full paralysis (pause of purposeful movements) and recovery (retrieval of purposeful movements/climbing) were recorded.
Full methods and any associated references are available in Supplementary Materials and Methods.
3.1. Multigenerational Developmentally Nonlethal Proteasome Inhibition in Drosophila Flies Increased Proteome Instability and Redox Imbalance: It Also Tended to Reduce Fecundity and Body Size, Caused Neuromuscular Defects, and Accelerated Aging
For this project, OregonRD. melanogaster flies were cultured continuously for more than 80 generations in the presence of 0.5 μΜ BTZ (G80-BTZ flies); this concentration of BTZ was chosen since it does not induce detrimental mortality during development . We also used G80-BTZ flies cultured in SM (G80 flies) or in 1 μM BTZ (G80-BTZ/1), as well as parental nontreated (NT) flies transiently exposed to 0.5 (NT-BTZ) or 1 μM (NT-BTZ/1) BTZ (Fig. S1).
As expected, G80-BTZ flies had lower proteasomal activities compared to NT flies (Fig. S2A); also, G80-BTZ flies’ tissues accumulated ubiquitinated (Fig. S2B1) and carbonylated (Fig. S2B2) polypeptides, as well as reactive oxygen species (ROS) (Fig. S2C), indicating the existence (vs. NT flies) of extensive proteome instability and redox imbalance.
Our analyses also revealed that chronic proteotoxic stress decreased the rate of egg-laying (Figure 1(a)) and hatching of adult flies (Figure 1(b)) in G80-BTZ vs. NT flies; this effect was evident even when G80-BTZ flies were compared to NT-BTZ flies and was also retained in G80 flies (Figures 1(a) and 1(b)), indicating permanent adaptations (a carry-over effect) in G80-BTZ flies’ physiology and associated molecular responses. Furthermore, G80-BTZ flies showed sex-independent reduced (compared to NT flies) wing area (Figure 1(c1 and c2); indicative of body size) and body weight values (Figure 1(c3)) along with decreased neuromuscular activity (Figure 1(d)). G80-BTZ female and male flies had a significant reduction (vs. NT flies) in their longevity (Figure 1(e1); Table S1). This accelerated aging effect was also evident in G80 flies (Figure 1(e2); Table S1) further supporting the notion of permanent alterations in flies’ physiology and in reduced survival rates after multigenerational proteotoxic stress.
3.2. G80-BTZ Flies Were Mildly Resistant to BTZ and Showed No Age-Related Loss of Proteasome Activity; These Adaptations Likely Relate to Sustained Upregulation of Proteostatic Modules
G80-BTZ flies have acquired a moderate resistance to increasing doses of BTZ on the contrary to NT flies, in which exposure to 0.5 and 1 μM of the inhibitor promoted a gradual decrease of proteasomal activities; this effect was not evident in G80-BTZ/1 vs. G80-BTZ flies (Figure 2(a)). In support, G80, G80-BTZ, and G80BTZ/1 flies showed no major differences in the levels of somatic tissue proteome ubiquitination (Figure 2(b)). Morphological studies in larvae and pupae revealed that exposure to 1 μΜ BTZ significantly reduced (compared to treatment with 0.5 μΜ BTZ) the size of NT-BTZ larvae and pupae, whereas the size of G80-BTZ larvae and pupae remained virtually unaffected following exposure to 1 μΜ BTZ (Figure 2(c)). In support, G80-BTZ/1 flies lived longer as compared to NT-BTZ/1 flies, with the most resistant being the G80-BTZ/1 female insects (Figure 2(d); Table S1). Notably, the acquired mild resistance to BTZ was not evident in higher BTZ doses (not shown).
Then, we asked whether the observed mild tolerance of G80-BTZ flies to higher BTZ doses is caused by alternations in the structure of the targeted proteasomes, which reduce the binding affinity of the inhibitor. By measuring the dose-dependent effect of BTZ on the activity of proteasomes isolated from NT and G80-BTZ flies, we found no differences in the level of inhibition (Fig. S3A); thus, the hypothesis for proteasomal structural changes (e.g., due to acquired mutations) that might have triggered the mild resistance to BTZ was discarded. In support, prolonged culturing of G80 flies in BTZ-free medium restored normal proteasomal peptidases’ activities (Fig. S3B), despite sustained proteome overubiquitination (Figure 2(b)) and redox imbalance (Fig. S3C).
Multigenerational proteome instability promoted the induction (vs. NT flies) of 19S (Rpn11, Rpn10, and Rpn6) and 20S (Prosα7, Prosβ5, Prosβ2, and Prosβ1) proteasomal genes (Figure 3(a)) and of proteasomal protein subunits (20S-α and Prosβ5) (Figure 3(b)) in G80 and G80-BTZ flies. The induction of proteostatic modules was likely permanent, as G80-BTZ flies did not show any age-dependent reduction of proteasomal activities (Figure 3(c)), which is a hallmark of aging in NT flies ; this adaptation has likely occurred at the cost of reduced responsiveness (i.e., proteasomal subunit upregulation; ) to increased doses of BTZ (Figure 3(b)). Furthermore, young (Figure 3(d1)) and aged (Fig. S4A) G80-BTZ flies expressed higher (compared to NT) levels of the autophagic ref(2)P (the fly ortholog of mammalian SQSTM1/p62), Atg6, and Atg8a genes; showed increased lysosomal staining in larva fat bodies (Fig. S4B), and both the G80 and G80-BTZ flies had upregulated activities of the lysosomal cathepsins B, L (Figure 3(d2)). Also, the cathD gene was induced (vs. NT flies) in G80-BTZ aged flies (Fig. S4A). Consistently, the G80-BTZ flies were more sensitive (compared to NT flies) to chloroquine (CQ) (an autophagy inhibitor) (Figure 3(e)) indicating that G80-BTZ flies are increasingly dependent to ALP for survival.
Thus, multigenerational exposure to proteotoxic stress caused the upregulation of proteostatic modules at the cost of reduced responsiveness to increased stress.
3.3. Multigenerational Proteome Instability in G80-BTZ Flies Induced Metabolic Reprogramming Being Evidenced by Increased Mitochondrial Biogenesis and Suppressed Insulin/IGF-Like Signaling (IIS)
Since IIS is implicated in the development of acquired resistance to BTZ in the clinic [31, 32], we then investigated whether the recorded adaptations in G80-BTZ flies were also associated with metabolic alterations. Confocal laser scanning microscopy (CLSM) analyses showed that multigenerational proteotoxic stress increased the number of mitochondria in the muscles of female and male G80-BTZ flies compared to NT (Figure 4(a)). This finding was in line with increased expression of the biogenesis-related mitochondrial genes PGC-1 and TFAM (Figure 4(b)); we also noted the upregulation of mitochondrial chaperone genes (Figure 4(b)) consistent with reduced mitochondrial proteome carbonylation and ubiquitination (Fig. S5). In addition, G80-BTZ flies showed reduced expression levels of the Opa1 (involved in fusion of mitochondrial inner membrane, cristae remodeling, and energetics regulation) and Pink1 (mitophagy-related) genes and expressed higher levels of the ATPsynβ gene (Figure 4(c1)); also, G80-BTZ females tend (not reaching statistical significance) to express higher levels of the ATP synthase dimers and oligomers (Figure 4(c2)). In support, G80-BTZ flies showed a trend (not statistically significant) towards increased mitochondrial respiration rates (Figure 4(d)). Thus, chronic proteotoxicity affected mitostatic pathways.
As mitochondrial function is tightly related to metabolic pathways’ regulation, we also studied sugar and lipid metabolism in G80-BTZ flies’ tissues. We found reduced (vs. NT flies) levels of glucose (GLU) and glycogen (GLY), along with a tendency (not statistically significant) for increased Trehalose (TREH) levels in male flies (Figure 5(a)) indicating a likely hyperglycemic state; TREH is the circulating sugar in flies’ haemolymph being synthesized in the fat body (the equivalent of mammalian liver and adipose tissue in flies). In support, we noted reduced GLY staining in the fat body of adult flies and a tendency for increased lipolysis in G80-BTZ flies’ fat body (Figure 5(b)). The noted metabolic adaptations were also evident at the genomic level as we found the upregulation of the Akt1, PyK (involved in glycolysis), and InR (insulin-like receptor) genes, along with the downregulation of the Pdk1 (involved in Krebs cycle regulation) and GlyP (promotes GLY degradation) genes in G80-BTZ vs. NT young flies; in middle-aged flies, we also observed the suppression of the Ilp2 gene (Figure 5(c)). Furthermore, we observed (mostly in female G80-BTZ flies) reduced expression of the inhibitory S21/S9 phosphorylated form of sgg/GSK3 (shaggy, the fly ortholog of mammalian glycogen synthase kinase-3) and increased foxo expression (Figure 5(d1)). We also observed the downregulation of the Ilp2 protein (ortholog to mammalian insulin; secreted from insulin-producing brain cells by cell-autonomous GLU sensing) in both the haemolymph (Figure 5(d2)) and the head tissue (Figure 5(d3)) of G80-BTZ flies; consistently, the Ilp2 gene was downregulated in G80-BTZ flies (Figure 5(c)). Thus, multigenerational proteotoxic stress suppressed IIS (Figure 5(e)). In line with these findings, ImpL2 (a muscle-secreted factor that inhibits Ilp2 activity) expression levels were elevated in G80-BTZ flies (Figure 5(d3)) and thus, the observed upregulation of InR and Akt1 genes (Figure 5(c)) is likely a late compensatory response due to suppressed IIS.
Taken together, these findings suggest that chronic proteome instability triggered metabolic reprogramming that suppressed IIS in adapted flies, indicating that prolonged stress signaling reallocates resources from growth and longevity to somatic preservation and stress tolerance.
3.4. Multigenerational Proteotoxic Stress-Mediated Toxicity Can Be Partially Mitigated by a Low-Protein Diet; yet, G80-BTZ Flies Were Increasingly Sensitive to Thermal Stress
IIS suppression is likely a prosurvival adaptation in G80-BTZ flies, which (among others) culminates in ALP activation (Figures 3(d), S4; see also, Figure 5(e)), a pathway shown before to correlate with increased longevity . In support, exposure of G80-BTZ flies to a low-protein-content diet tended (not reaching statistical significance) to extend the median longevity of G80-BTZ flies (Figure 6(a1); Table S1). A high-protein or a low-calories diet was toxic in G80-BTZ flies (Fig. S6), while no significant effects on longevity were found in parental NT or NT-BTZ flies fed with low-protein-content diet (Fig. S7). Interestingly, apart from the acquired differences in the basal expression levels of the autophagic ref(2)P and Atg8a genes, low-protein-content diet did not induce significantly distinct responses in ref(2)P and Atg8a genes in the NT and G80-BTZ flies’ populations (Figure 6(a2)) indicating that other pathways likely contribute to increased longevity of low-protein diet-fed G80-BTZ flies.
The acquired sustained suppression of IIS in G80-BTZ flies would also result in decreased activity of cytoprotective Nrf2 (Figure 5(e)). Indeed, treatment of G80-BTZ flies with 6-bromo-indirubin-3-oxime (6BIO, a selective sgg/GSK3 inhibitor)  resulted in increased expression levels of cncC (the fly ortholog of mammalian Nrf2) transcriptional targets (Figure 6(b)). This observation would then indicate enhanced sensitivity of G80-BTZ flies to additional and/or to other types of proteome instability-inducing stress factors. Consistently, G80-BTZ flies were increasingly susceptible in a sex-independent manner to thermal stress as they were paralyzed faster (Figure 6(c1)) and recovered with a slower rate (Figure 6(c2)) vs. NT flies after exposure to heat shock, whereas they were unaffected by mechanical stress (Figure 6(d)).
Therefore, the trade-off adaptations under conditions of chronic proteotoxic stress occur at the cost of reduced tolerance to additional proteotoxic stress illustrating the limited buffering capacity of higher metazoan survival pathways.
As acquired resistance to BTZ is often seen in the clinic in treated myeloma patients , and proteinopathies (such as neurodegenerative disorders) show high prevalence due to increased aging of the population , gaining insights into the molecular adaptations triggered at the organismal level due to chronic proteome instability is paramount for the development of complementary or alternative treatments for these devastating diseases. By developing the first in vivo model of multigenerational (>80 generations/>4 years) developmentally nonlethal proteome instability (also accompanied by redox imbalance) in Drosophila flies, we aimed to understand how proteotoxic stress acts on the evolutionary dynamics of populations and shapes stress response(s) across generations. Our findings indicate that chronic proteotoxic stress triggers a series of adaptations on flies’ physiology including reduced fecundity and body size, locomotion defects, and accelerated aging. Similarly, to our findings, exposure of Caenorhabditis elegans to low concentration of bisphenol A (an organic synthetic compound exhibiting estrogen-mimicking properties) across four generations resulted in individuals that grew smaller, moved slower, and produced less offspring as compared to controls . Also, exposure of Daphnia magna to elevated temperature for three generations significantly decreased the offspring’s number, the time to first brood, and the body length compared to animals grown under optimal temperature; these effects were accompanied by increased ROS and lipid peroxidation .
Although studies in nonmyeloma or myeloma cell lines have suggested that acquired resistance enhanced proteasome activity and/or promoted mutations in the CT-L-related β5 proteasomal subunit that impaired BTZ binding [19, 37]; mutations have not been detected in the PSMB5 gene of multiple myeloma (MM) patients being refractory to or relapsed from BTZ treatment [24, 25]. In support, we found no impairment of BTZ binding in isolated proteasomes from G80-BTZ flies, in which the acquired mild resistance to BTZ likely relates to sustained upregulation of proteostatic modules; these adaptations were largely evident in G80 flies suggesting a carry-over effect. Indeed, it has been found that upon reversion to standard nutrition, flies whose prior generations have been exposed to a high-protein diet displayed multigenerational inheritance of altered gene expression . Moreover, populations of Drosophila subobscura retain signatures from past contamination events with heavy metals , while exposure to G418 stress reduces the maternal levels of polycomb in the offspring embryos; this reduction contributed to the inheritance of induced gene expression patterns .
In parallel, we noted increased activities of lysosomal cathepsins and autophagic genes which along with increased sensitivity of G80-BTZ flies to CQ suggested that they gradually became dependent for their survival to higher ALP activities. Consistently, inhibition of ALP with CQ potentiates carfilzomib-induced apoptosis in myeloma cells in vitro and in vivo . Also, in accordance with our findings, other reported mechanisms of acquired resistance to BTZ involve reprogramming of the Nrf2 pathway or IIS and/or upregulation of the heat shock response signaling pathway .
These prosurvival adaptations in G80-BTZ flies likely occurred at the cost of minimal responses of proteostatic modules to increased BTZ doses and increased susceptibility to various types of additional proteotoxic stress, namely, autophagy inhibition or thermal stress. Consistently, it was found that recurrent stress across three generations of female rats may cumulatively increase stress vulnerability and the risk of adverse health outcomes through perinatal programming . In general, it is assumed that the noted adaptations relate to epigenetic effects; for instance, DNA hypomethylation of inflammation-associated genes in the adipose tissue has been described as an effect of multigenerational high-protein feeding in female mice , while increased expression of Hsp70 accompanied by changes in histone H3 methylation and histone H4 acetylation has been observed in Artemia salina (brine shrimp) after exposure to thermal stress . Similarly, it was proposed that the evolution of insecticide resistance results from epigenetic modifications, which are heritable and influence gene expression without changing the underlying DNA sequence .
Interestingly, multigenerational proteome instability and redox imbalance in G80-BTZ flies caused metabolic reprogramming being evidenced by increased mitochondrial biogenesis and suppressed insulin/IGF-like signaling (IIS); consistently, we recently found that transient proteasome dysfunction disrupted mitochondrial morphology and function [8, 46]. Altered mental health through metabolic pathways’ reprogramming has been documented after exposure of four generations of rats during pregnancy to stress . Beyond energetics, mitochondria are also central executors of apoptosis; thus, altered mitochondrial function could infer changes to apoptosis process. Indeed, BTZ-resistant cells had mitochondrial adaptations that minimized induction of apoptosis , while induction of mitochondrial biogenesis enhanced resistance to several apoptotic stimuli in myocytes .
Prolonged stress signaling seems to be centrally linked to IIS downregulation since genotoxic stress in XPF-ERCC1-deficient mice reallocates resources from growth to the somatic preservation and life extension ; similarly, multigenerational proteotoxic stress induced, mainly in female flies, the downregulation of IIS. It has been reported that IIS is implicated in the development of acquired resistance to BTZ since IGF-1 enhanced the cytotoxic effect of proteasome inhibitors . Therefore, IIS suppression is likely a prosurvival adaptation in G80-BTZ flies, which (among others) activated ALP, a pathway shown before to correlate with increased longevity ; thus, it is not surprising that exposure of G80-BTZ flies to low-protein diet tends to increase their median longevity.
Overall, our presented data highlight the toxic effects of multigenerational proteotoxic stress and the extensive functional wiring of proteostatic and metabolic/energetic pathways, indicating also that higher metazoans maximize fitness by adopting prosurvival alterations in proteostatic-mitostatic-metabolic pathways in response to prolonged proteome instability. Part of these adaptations to chronic proteotoxic stress includes a highly conserved adaptive metabolic response mediated by the IIS pathway, which reallocates resources from growth and longevity to somatic preservation and stress tolerance. Yet, these constraints and trade-off adaptations take place at the cost of accelerated aging and/or reduced tolerance to additional stress illustrating the exhaustion of the survival pathway buffering capacity.
The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
Conflicts of Interest
The authors declare no conflict of interest.
IPT designed and supervised the study; MSM, ENT, and IPT conducted the experiments or interpreted the data; and IPT wrote the manuscript. Maria S. Manola and Eleni N. Tsakiri contributed equally to this work.
We thank Prof. Maria Figueiredo-Pereira (Hunter College, NY, USA) for the β5 antibody, Ernst Hafen (Institute of Molecular System Biology, ETH Zurich, Switzerland) for the Ilp2 and ImpL2 antibodies, Otto Baba (Ohu University, Japan) for the anti-GLY antibody, and Prof. Alexios-Leandros Skaltsounis (Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece) for 6BIO. IPT acknowledges funding from the EU Project TASCMAR (EU-H2020/634674) and the Hellenic GSRT Project BIOIMAGING-GR (MIS 5002755). MM is a recipient of a fellowship from the Hellenic State Scholarships Foundation (IKY). This research is cofinanced by Greece and the European Union (European Social Fund (ESF)) through the Operational Programme “Human Resources Development, Education and Lifelong Learning” in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (ΙΚY).
Supplementary Information includes figures and associated legends; Table S1 (longevity assay statistics); list of abbreviations; Materials and Methods; and associated References. (Supplementary Materials)
- I. P. Trougakos, F. Sesti, E. Tsakiri, and V. G. Gorgoulis, “Non-enzymatic post-translational protein modifications and proteostasis network deregulation in carcinogenesis,” Journal of Proteomics, vol. 92, pp. 274–298, 2013.
- C. L. Klaips, G. G. Jayaraj, and F. U. Hartl, “Pathways of cellular proteostasis in aging and disease,” The Journal of Cell Biology, vol. 217, no. 1, pp. 51–63, 2018.
- J. Labbadia and R. I. Morimoto, “The biology of proteostasis in aging and disease,” Annual Review of Biochemistry, vol. 84, no. 1, pp. 435–464, 2015.
- S. Kaushik and A. M. Cuervo, “Proteostasis and aging,” Nature Medicine, vol. 21, no. 12, pp. 1406–1415, 2015.
- E. N. Tsakiri and I. P. Trougakos, “The amazing ubiquitin-proteasome system: structural components and implication in aging,” International Review of Cell and Molecular Biology, vol. 314, pp. 171–237, 2015.
- D. J. Klionsky, K. Abdelmohsen, A. Abe et al., “Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition),” Autophagy, vol. 12, no. 1, pp. 1–222, 2016.
- G. P. Sykiotis and D. Bohmann, “Stress-activated cap'n'collar transcription factors in aging and human disease,” Science Signaling, vol. 3, no. 112, article re3, 2010.
- E. N. Tsakiri, S. Gumeni, K. K. Iliaki et al., “Hyperactivation of Nrf2 increases stress tolerance at the cost of aging acceleration due to metabolic deregulation,” Aging Cell, vol. 18, article e12845, 2018.
- A. E. Webb and A. Brunet, “FOXO transcription factors: key regulators of cellular quality control,” Trends in Biochemical Sciences, vol. 39, no. 4, pp. 159–169, 2014.
- L. R. Lapierre, C. Kumsta, M. Sandri, A. Ballabio, and M. Hansen, “Transcriptional and epigenetic regulation of autophagy in aging,” Autophagy, vol. 11, no. 6, pp. 867–880, 2015.
- D. Finley, X. Chen, and K. J. Walters, “Gates, channels, and switches: elements of the proteasome machine,” Trends in Biochemical Sciences, vol. 41, no. 1, pp. 77–93, 2016.
- A. Ciechanover, A. Orian, and A. L. Schwartz, “The ubiquitin-mediated proteolytic pathway: mode of action and clinical implications,” Journal of Cellular Biochemistry, vol. 77, no. S34, pp. 40–51, 2000.
- A. Höhn and T. Grune, “Lipofuscin: formation, effects and role of macroautophagy,” Redox Biology, vol. 1, no. 1, pp. 140–144, 2013.
- Y. Medkour, V. Svistkova, and V. I. Titorenko, “Cell-nonautonomous mechanisms underlying cellular and organismal aging,” International Review of Cell and Molecular Biology, vol. 321, pp. 259–297, 2016.
- R. Taylor and A. Dillin, “XBP-1 is a cell-nonautonomous regulator of stress resistance and longevity,” Cell, vol. 153, no. 7, pp. 1435–1447, 2013.
- C. López-Otín, M. A. Blasco, L. Partridge, M. Serrano, and G. Kroemer, “The hallmarks of aging,” Cell, vol. 153, no. 6, pp. 1194–1217, 2013.
- V. G. Gorgoulis, D. E. Pefani, I. S. Pateras, and I. P. Trougakos, “Integrating the DNA damage and protein stress responses during cancer development and treatment,” The Journal of Pathology, vol. 246, no. 1, pp. 12–40, 2018, Epub ahead of print.
- M. A. Dimopoulos, P. Moreau, A. Palumbo et al., “Carfilzomib and dexamethasone versus bortezomib and dexamethasone for patients with relapsed or refractory multiple myeloma (ENDEAVOR): a randomised, phase 3, open-label, multicentre study,” The Lancet Oncology, vol. 17, no. 1, pp. 27–38, 2016.
- E. E. Manasanch and R. Z. Orlowski, “Proteasome inhibitors in cancer therapy,” Nature Reviews Clinical Oncology, vol. 14, no. 7, pp. 417–433, 2017.
- J. M. Steele, “Carfilzomib: a new proteasome inhibitor for relapsed or refractory multiple myeloma,” Journal of Oncology Pharmacy Practice, vol. 19, no. 4, pp. 348–354, 2013.
- E. N. Tsakiri, E. Terpos, E. D. Papanagnou et al., “Milder degenerative effects of carfilzomib vs. bortezomib in the Drosophila model: a link to clinical adverse events,” Scientific Reports, vol. 7, no. 1, article 17802, 2017.
- E. D. Papanagnou, E. Terpos, E. Kastritis et al., “Molecular responses to therapeutic proteasome inhibitors in multiple myeloma patients are donor-, cell type- and drug-dependent,” Oncotarget, vol. 9, no. 25, pp. 17797–17809, 2018.
- D. Chen, M. Frezza, S. Schmitt, J. Kanwar, and Q. P. Dou, “Bortezomib as the first proteasome inhibitor anticancer drug: current status and future perspectives,” Current Cancer Drug Targets, vol. 11, no. 3, pp. 239–253, 2011.
- M. Politou, A. Karadimitris, E. Terpos, I. Kotsianidis, J. F. Apperley, and A. Rahemtulla, “No evidence of mutations of the PSMB5 (beta-5 subunit of proteasome) in a case of myeloma with clinical resistance to bortezomib,” Leukemia Research, vol. 30, no. 2, pp. 240-241, 2006.
- L. Shuqing, Y. Jianmin, H. Chongmei, C. Hui, and J. Wang, “Upregulated expression of the PSMB5 gene may contribute to drug resistance in patient with multiple myeloma when treated with bortezomib-based regimen,” Experimental Hematology, vol. 39, no. 12, pp. 1117-1118, 2011.
- E. N. Tsakiri, G. P. Sykiotis, I. S. Papassideri, V. G. Gorgoulis, D. Bohmann, and I. P. Trougakos, “Differential regulation of proteasome functionality in reproductive vs. somatic tissues of Drosophila during aging or oxidative stress,” The FASEB Journal, vol. 27, no. 6, pp. 2407–2420, 2013.
- I. P. Trougakos and L. H. Margaritis, “Immunolocalization of the temporally "early" secreted major structural chorion proteins, Dvs38 and Dvs36, in the eggshell layers and regions of Drosophila virilis,” Journal of Structural Biology, vol. 123, no. 2, pp. 111–123, 1998.
- M. Y. Davis, K. Trinh, R. E. Thomas et al., “Glucocerebrosidase deficiency in Drosophila results in α-synuclein-independent protein aggregation and neurodegeneration,” PLoS Genetics, vol. 12, no. 3, article e1005944, 2016.
- H. Zhang, J. Tan, E. Reynolds, D. Kuebler, S. Faulhaber, and M. Tanouye, “The Drosophila slamdance gene: a mutation in an aminopeptidase can cause seizure, paralysis and neuronal failure,” Genetics, vol. 162, no. 3, pp. 1283–1299, 2002.
- E. N. Tsakiri, G. P. Sykiotis, I. S. Papassideri et al., “Proteasome dysfunction in Drosophila signals to an Nrf2-dependent regulatory circuit aiming to restore proteostasis and prevent premature aging,” Aging Cell, vol. 12, no. 5, pp. 802–813, 2013.
- D. J. Kuhn, Z. Berkova, R. J. Jones et al., “Targeting the insulin-like growth factor-1 receptor to overcome bortezomib resistance in preclinical models of multiple myeloma,” Blood, vol. 120, no. 16, pp. 3260–3270, 2012.
- I. Tagoug, L. P. Jordheim, S. Herveau et al., “Therapeutic enhancement of ER stress by insulin-like growth factor I sensitizes myeloma cells to proteasomal inhibitors,” Clinical Cancer Research, vol. 19, no. 13, pp. 3556–3566, 2013.
- E. N. Tsakiri, N. Gaboriaud-Kolar, K. K. Iliaki et al., “The indirubin derivative 6-bromoindirubin-3-oxime activates proteostatic modules, reprograms cellular bioenergetic pathways, and exerts antiaging effects,” Antioxidants & Redox Signaling, vol. 27, no. 14, pp. 1027–1047, 2017.
- M. A. Dimopoulos, P. G. Richardson, P. Moreau, and K. C. Anderson, “Current treatment landscape for relapsed and/or refractory multiple myeloma,” Nature Reviews Clinical Oncology, vol. 12, no. 1, pp. 42–54, 2015.
- D. Zhou, J. Yang, H. Li, Q. Lu, Y. D. Liu, and K. F. Lin, “Ecotoxicity of bisphenol A to Caenorhabditis elegans by multigenerational exposure and variations of stress response in vivo across generations,” Environmental Pollution, vol. 208, Part B, pp. 767–773, 2016.
- E. Bae, P. Samanta, J. Yoo, and J. Jung, “Effects of multigenerational exposure to elevated temperature on reproduction, oxidative stress, and Cu toxicity in Daphnia magna,” Ecotoxicology and Environmental Safety, vol. 132, pp. 366–371, 2016.
- N. E. Franke, D. Niewerth, Y. G. Assaraf et al., “Impaired bortezomib binding to mutant β5 subunit of the proteasome is the underlying basis for bortezomib resistance in leukemia cells,” Leukemia, vol. 26, no. 4, pp. 757–768, 2012.
- A. J. Osborne and P. K. Dearden, “A ‘phenotypic hangover’: the predictive adaptive response and multigenerational effects of altered nutrition on the transcriptome of Drosophila melanogaster,” Environmental Epigenetics, vol. 3, no. 4, article dvx019, 2017.
- B. Kenig, Z. Kurbalija Novičić, A. Patenković, M. Stamenković-Radak, and M. Anđelković, “Adaptive role of inversion polymorphism of Drosophila subobscura in lead stressed environment,” PLoS One, vol. 10, no. 6, article e0131270, 2015.
- S. Stern, O. Snir, E. Mizrachi, M. Galili, I. Zaltsman, and Y. Soen, “Reduction in maternal Polycomb levels contributes to transgenerational inheritance of a response to toxic stress in flies,” The Journal of Physiology, vol. 592, no. 11, pp. 2343–2355, 2014.
- V. Jarauta, P. Jaime, O. Gonzalo et al., “Inhibition of autophagy with chloroquine potentiates carfilzomib-induced apoptosis in myeloma cells in vitro and in vivo,” Cancer Letters, vol. 382, no. 1, pp. 1–10, 2016.
- J. K. McCreary, L. S. Truica, B. Friesen et al., “Altered brain morphology and functional connectivity reflect a vulnerable affective state after cumulative multigenerational stress in rats,” Neuroscience, vol. 330, pp. 79–89, 2016.
- Y. Ding, J. Li, S. Liu et al., “DNA hypomethylation of inflammation-associated genes in adipose tissue of female mice after multigenerational high fat diet feeding,” International Journal of Obesity, vol. 38, no. 2, pp. 198–204, 2014.
- P. Norouzitallab, K. Baruah, M. Vandegehuchte et al., “Environmental heat stress induces epigenetic transgenerational inheritance of robustness in parthenogenetic Artemia model,” The FASEB Journal, vol. 28, no. 8, pp. 3552–3563, 2014.
- K. Brevik, L. Lindström, S. D. McKay, and Y. H. Chen, “Transgenerational effects of insecticides — implications for rapid pest evolution in agroecosystems,” Current Opinion in Insect Science, vol. 26, pp. 34–40, 2018.
- E. N. Tsakiri, S. Gumeni, K. K. Iliaki et al., “Proteasome dysfunction induces excessive proteome instability and loss of mitostasis that can be mitigated by enhancing mitochondrial fusion or autophagy,” Autophagy, pp. 1–17, 2019.
- D. Kiss, M. Ambeskovic, T. Montina, and G. A. S. Metz, “Stress transgenerationally programs metabolic pathways linked to altered mental health,” Cellular and Molecular Life Sciences, vol. 73, no. 23, pp. 4547–4557, 2016.
- I. S. Song, H. K. Kim, S. R. Lee et al., “Mitochondrial modulation decreases the bortezomib-resistance in multiple myeloma cells,” International Journal of Cancer, vol. 133, no. 6, pp. 1357–1367, 2013.
- A. D. Dam, A. S. Mitchell, and J. Quadrilatero, “Induction of mitochondrial biogenesis protects against caspase-dependent and caspase-independent apoptosis in L6 myoblasts,” Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, vol. 1833, no. 12, pp. 3426–3435, 2013.
- L. J. Niedernhofer, G. A. Garinis, A. Raams et al., “A new progeroid syndrome reveals that genotoxic stress suppresses the somatotroph axis,” Nature, vol. 444, no. 7122, pp. 1038–1043, 2006.
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