Increased Gene Expression of RUNX2 and SOX9 in Mesenchymal Circulating Progenitors Is Associated with Autophagy during Physical Activity
Physical exercise improves chondrogenic differentiation and affects adipogenic differentiation. Besides SOX9 upregulation, we observed also a higher expression of COL2A1 and COMP in M-CPCs after the run (a). In vitro experiments showed an increased chondrogenic differentiation in the MSC line cultured in the presence of POST RUN sera. In fact, a higher expression of chondrogenic genes (b) and of Aggrecan protein (c) as well as more intense Alcian blue staining ((d); 10x) was observed. Adipogenic differentiation. PPARG2 gene expression was higher in POST RUN M-CPCs (e). Conversely, the MSC line treated with POST RUN sera showed reduced adipogenic differentiation. In fact, PPARG2 gene expression levels were lower (f); the number of lipid droplets ((g); 10x) and of PLIN1-positive cells ((h); 40x) as well as PLIN1 protein levels (i) was reduced in the MSC line treated with POST RUN sera. The in vitro analyses were performed in three independent experiments. ;#.
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