Metformin Improves Fertility in Obese Males by Alleviating Oxidative Stress-Induced Blood-Testis Barrier Damage

Table 2

Primers for real-time PCR detection.

Gene	Species	Forward primer	Reverse primer
ZO-1	Mouse	GCCGCTAAGAGCACAGCAA	TCCCCACTCTGAAAATGAGGA
Occludin	Mouse	TTGAAAGTCCACCTCCTTACAGA	CCGGATAAAAAGAGTACGCTGG
N-cadherin	Mouse	AGCGCAGTCTTACCGAAGG	TCGCTGCTTTCATACTGAACTTT
beta-Catenin	Mouse	CCCAGTCCTTCACGCAAGAG	CATCTAGCGTCTCAGGGAACA
Nectin2	Mouse	GCATCATTGGAGGTATTATCGCT	GAGGGAGGTCCTTCCAGTTC
NF-κB/p65	Mouse	CGGGATGGCTACTATGAGGC	CGTGAAAGGGGTTATTGTTGGT
beta-Actin	Mouse	GGCTGTATTCCCCTCCATCG	CCAGTTGGTAACAATGCCATGT