Hsp90 Inhibitor SNX-2112 Enhances TRAIL-Induced Apoptosis of Human Cervical Cancer Cells via the ROS-Mediated JNK-p53-Autophagy-DR5 Pathway

Figure 2

SNX-2112 is synergistic to TRAIL and coinduces HeLa cell apoptosis. (a-i) Cells were treated with either TRAIL (200 ng/mL) or SNX-2112 (125 nM) alone or in combination for 48 h. Using FACS analysis assays, the cells were pretreated with or without 20 μM Z-VAD-fmk for 1 h. (a, b) Microscopic cell morphologies. Scale bar: 250 μm. Morphologically altered cells were counted and analyzed. (c, d) The cells were stained with Annexin V and propidium iodide (PI), followed by FACS analysis. (e, f) FACS analysis of by JC-1 staining. After treatment with drugs, cells were stained with JC-1 for 20 min. (g h) The Bcl-2, Bcl-XL, FLIP, pro-caspase 3, c-caspase 3 and 8, and c-PARP proteins were analyzed by Western blotting. (i) Apoptosis-associated proteins (c-caspase 3 and 8 and c-PARP) were detected in the presence of Z-VAD-fmk by Western blotting. GAPDH was used as a protein loading control. Data are represented as . Error bars represent SD from three separate experiments. and compared with the control group.

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