Hsp90 Inhibitor SNX-2112 Enhances TRAIL-Induced Apoptosis of Human Cervical Cancer Cells via the ROS-Mediated JNK-p53-Autophagy-DR5 Pathway

Figure 7

SNX-2112-induced JNK activation is involved in apoptosis induced by TRAIL/SNX-2112. (a, b) HeLa cells were treated with SNX-2112 at indicated doses for 6 h. Western blotting was performed to detect the levels of p-ERK, ERK, p-JNK, JNK, p-p38, and p38. Densitometry analyses of the bands for each protein were performed. HeLa cells were pretreated with (c, d) 10 μM SP600125, (e, f) 25 μM PD98059, and (g, h) 10 μM SB203580 for 2 h and then treated with either TRAIL (200 ng/mL) or SNX-2112 (125 nM) alone or in combination for 48 h. TRAIL/SNX-2112-induced apoptosis was analyzed by flow cytometry. (i, j) HeLa cells were pretreated with or without 10 mM SP600125 for 2 h and then treated with or without SNX-2112 for 48 h. Western blotting were used to analyze whole-cell extracts and detect the levels of p53, LC3, and DR5. Densitometry analyses of the bands for each protein were performed. Data are represented as . Error bars represent SD from three separate experiments. and compared with the control group.

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