Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 2

Research Article

Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells

Figure 2

MGO treatment enhances autophagy. (a) Expression of autophagy-related proteins in HK-2 cells treated with MGO (0.5–0.75 mM) for 24 h. (b) The time-dependent protein expression levels of LC3 and p62 in HK-2 cells treated with MGO (0.75 mM) and torin 1 (positive control, 200 nM, 4 h) with or without Baf (50 nM, 1 h). (c) Transcriptional levels of p62/SQSTM-1 mRNA. (d) Representative immunofluorescence image of HK-2 cells treated with MGO for 24 h (green: LC3; blue: DAPI; original magnification: 600x). (e) LC3 puncta following treatment with MGO. Values are . and versus vehicle (0) group.

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