Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells

Figure 5

MGO upregulated the expression of autophagy-related transcription factors by enhancing AMPK activity. (a) Time-dependent mRNA levels of autophagy-related transcription factors induced by 0.75 mM of MGO. (b) mRNA levels of autophagy subunits modulated by transcription factors in HK-2 cells treated with MGO (0.75 mM). (c) Transcription factor mRNA levels of transient siAMPK-transfected cells compared with those of control cells treated with MGO for 8 h. (d) Expression of MAP1LC3B mRNA and LC3 protein level in siNC or siAMPK cells following treatment with MGO for 8 h (0.75 mM) or 24 h (0-0.75 mM), respectively. Values are . , , and versus the control (0) group; ^# and ^### versus the same concentration of MGO ( experiments).

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