Autophagy Functions to Prevent Methylglyoxal-Induced Apoptosis in HK-2 Cells
Table 1
Primer sequences.
DNA target
Sequence (5 to 3)
MAP1LC3B
F
GATGTCCGACTTATTCGAGAGC
R
TTGAGCTGTAAGCGCCTTCTA
BECN11
F
CCATGCAGGTGAGCTTCGT
R
GAATCTGCGAGAGACACCATC
FOXO3
F
CGGACAAACGGCTCACTCT
R
GGACCCGCATGAATCGACTAT
TFE3
F
CCGTGTTCGTGCTGTTGGA
R
GCTCGTAGAAGCTGTCAGGAT
TFEB
F
ACCTGTCCGAGACCTATGGG
R
CGTCCAGACGCATAATGTTGTC
FOXO1
F
TCGTCATAATCTGTCCCTACACA
R
CGGCTTCGGCTCTTAGCAAA
CREB
F
TCAGCCGGGTACTACCATTC
R
TCTCTTGCTGCTTCCCTGTT
GABARAB
F
AGAAGAGCATCCGTTCGAGAA
R
CCAGGTCTCCTATCCGAGCTT
ATG16L
F
AACGCTGTGCAGTTCAGTCC
R
AGCTGCTAAGAGGTAAGATCCA
BNIP3
F
CAGGGCTCCTGGGTAGAACT
R
CTACTCCGTCCAGACTCATGC
ACTIN
F
CATGTACGTTGCTATCCAGGC
R
CTCCTTAATGTCACGCACGAT
AMPKα
F
TTG AAA CCT GAA AAT GTC CTG CT
R
GGT GAG CCA CAA CTT GTT CTT
ATG5
F
TGTGCTTCGAGATGTGTGGTT
R
GTCAAATAGCTGACTCTTGGCAA
ATG12
F
TAGAGCAACACGAACCATCC
R
CACTGCCAAAACACTCATAGAGA
p62/SQSTM-1
F
GACTACGACTTGTGTAGCCTC
R
AGTGTCCGTGTTTCACCTTCC
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