Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells

Figure 2

VHL knockdown induced LCN2 secretion in HK-2 cells. HK-2 cells were transfected with a vector expressing a scrambled shRNA (SC) or an shRNA specific for VHL: shVHL1 (V1) or shVHL3 (V3), as described in a previous study (4) by electroporation (Gemini X2 System) and incubated for 48 hours. (a) The Western blot analysis of LCN2 using an anti-LCN2 antibody at 50 μg of protein/lane in the conditioned medium under the indicated conditions in the HK-2 cells. (b) The level of LCN2 was quantified using ImageJ. (c) The cell lysates were subject to Western blot analysis with the anti-LCN2 antibody. (d) An equal volume of 10 μL of urine samples from the wild-type and Vhl conditional knockout mice was analyzed by Western blot analysis using the anti-LCN2 antibody. The lower panel shows the level of LCN2 was quantified by ImageJ. All data are presented as the . and .

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