Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells

Figure 3

LCN2 was increased in Vhl mutant mRTCs. (a) Primary mouse renal tubule cells (mRTCs) were isolated from the wild-type Hoxb7-Cre-GFP/+ (W) or the mutant Hoxb7-Cre-GFP/+; Vhl^fl/fl (M) mice. The cell lysate was subject to Western blot analysis with the indicated antibodies. β-Actin was used as a loading control. The knockout of Vhl was confirmed by assaying the level of VHL (top). (b) The level of LCN2 was quantified using ImageJ. All data are presented as the . .

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