Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells

Figure 4

LCN2 knockdown attenuated the increase in ROS production in VHL knockdown HK-2 cells. (a) The change in the level of cellular ROS in HK-2 cells transfected with a vector expressing one scrambled shRNA (SC), two scrambled shRNAs (SC+SC), the scrambled shRNA (SC) and shRNA specific for LCN2 (L1) (SC+L1), the shRNA specific for VHL (V3) and scrambled shRNA (SC) (V3+SC), or the shRNA specific for VHL (V3) and shRNA specific for LCN2 (L1) (V3+L1). (b) ROS generation was expressed as mean fluorescence intensity. (c) The change in the level of cellular ROS in HK-2 cells transfected with a vector expressing the shRNA specific for VHL (V1) and scrambled shRNA (SC) (V1+SC) or shRNA specific for VHL (V1) and shRNA specific for LCN2 (L1) (V1+L1). (d) ROS generation was expressed as mean fluorescence intensity. All data are presented as the . .

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