Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells

<div><i>LCN2</i> knockdown attenuated the VHL inactivation-induced chemotaxis of macrophages. The HK-2 cells or without the knockdown of <i>VHL</i> (V3) or without (SC) and with knockdown of <i>LCN2</i> (L1) or without (SC) were assayed for their ability to recruit monocyte/macrophage RAW264.7 cells. (a) HK-2 cells transfected with vectors containing a scrambled shRNA sequence (SC), shVHL3 (V3), or shLCN2 (L1) were assayed for their respective capacity to induce macrophage chemotaxis for 48 hours. The macroscopic observation of the Transwell chambers was performed. (b) Migration in the Transwell system was allowed for 24 hours. The relative number of migrated cells stained by crystal violet was measured at OD<sub>570</sub>. 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Oxidative Medicine and Cellular Longevity

fig7

Figure 7

Figure 7: Loss of Function of von Hippel-Lindau Trigger Lipocalin 2-Dependent Inflammatory Responses in Cultured and Primary Renal Tubular Cells 