Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage

Figure 5

The SAH grading scores were similar among each surgery group (, Fig. 4(a), p =0.852), The neurological deficit scores among each surgery group (, Fig. 4(b), p =0.806) along with the evaluation of SAH grade. Treatment with 4 mg/kg ebselen to determine the EBI (c-e), 4 mg/kg ebselen decreased neurological function deficit compared with the SAH and Abs+SAH groups (c). BBB permeability was also alleviated in four areas (LH, RH, CB, and BS) after treatment with 4 mg/kg ebselen (d). Brain water content was decreased in four areas (LH, RH, CB, and BS) after treatment with 4 mg/kg ebselen (e), P <0.05 vs Abs+SAH group (, each group). Treatment with 5 mg/kg heparin to determine the EBI (f-h), 5 mg/kg heparin decreased neurological functions (f). BBB permeability based on Evans blue staining in the LH and RH was decreased by the 5 mg/kg heparin treatment (g). Brain water content was decreased in four areas (LH, RH, CB, and BS) after treatment with 5 mg/kg heparin (h), P <0.05 vs NS + SAH group (, each group). Treatment with 10 μg OSM to determine the EBI (i-k), 10 μg OSM aggravated neurological functions (i). BBB permeability based on Evans blue staining in the LH and RH was increased by the 10 μg OSM treatment (j). Brain water content was increased in four areas (LH, RH, CB, and BS) after treatment with 10 μg OSM (k), P <0.05 vs PBS + SAH group (, each group).

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