Research Article
PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway
Figure 5
PIN1 modulates HEI-OC1 senescence. (a, c) HEI-OC1 cells were transfected with the HA-PIN1 plasmid. (b, d) HEI-OC1 cells were treated by juglone. (e–h) Western blots for the expression of p53, p-p53, p21, and p16 in HEI-OC1 cells. (i, j) SA-β-gal staining was used to detect the senescent state of HEI-OC1 cells (OE: overexpression-PIN1; vs. control group, # vs. H2O2 group. ).
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