PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway

Figure 6

PIN1 mediates HEI-OC1 cell senescence by affecting the PI3K/Akt/mTOR signaling pathway. (a) Western blots for the expression of p-Akt, Akt, p-mTOR, and mTOR in HEI-OC1 cells after transfection with the HA-PIN1 plasmid. (b) Western blots for the expression of p-Akt, Akt, p-mTOR, mTOR, and PIN1 in HEI-OC1 cells after pretreatment with SC79 (Akt activator). (c) Western blots for the expression of p53, p-p53, p21, and p16 after pretreatment with SC79. (d) Western blots for the expression of p-Akt, Akt, p-mTOR, mTOR, and PIN1 in HEI-OC1 cells after pretreatment with LY294002 (an inhibitor of PI3K/Akt). (e) Western blots for the expression of p53, p-p53, p21, and p16 after pretreatment with LY294002. (f) SA-β-gal staining for HEI-OC1 cells ( vs. control group, ^# vs. DMSO+H₂O₂ group, ^& vs. H₂O₂ group. ).

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