PIN1 Protects Hair Cells and Auditory HEI-OC1 Cells against Senescence by Inhibiting the PI3K/Akt/mTOR Pathway

Figure 8

H₂O₂ affects the expression and activity of p53 through ROS and then affects senescence. (a, b) Intracellular ROS levels were measured by DCFH-DA in HEI-OC1 cells (ns: nonsignificant; vs. control group, ^# vs. H₂O₂ group). (c–f) Western blots for the expression of p53, p-p53, p21, p16, and PIN1 after pretreatment with the ROS inhibitor N-acetyl-L-cysteine (NAC) ( vs. the DMSO group, ^# vs. H₂O₂ group, ^& vs. the juglone group). (g, h) SA-β-gal staining for HEI-OC1 cells ( vs. the control group, ^# vs. the H₂O₂ group, ^& vs. the juglone group. ).

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