Immunomodulatory Role of Ocimum gratissimum and Ascorbic Acid against Nicotine-Induced Murine Peritoneal Macrophages In Vitro
Figure 2
Effects of nicotine (N) (10 mM), aqueous extract (Ae-Og) (10 μg/mL), and ascorbic acid (AA) (0.01 mM) on (a) adherence index, (b) chemotactic index, (c) phagocytic index, and (d) intracellular killing property of murine peritoneal macrophages are presented in Figure 2. After the treatment schedule, as mentioned in Section 4, the cells were collected and the functions of murine peritoneal macrophages were observed. Nicotine-treated cells showed the significant () decreased functional activity in time-dependent manner, which were significantly increased during supplementation of Ae-Og and ascorbic acid. All the experimental results are presented as mean ± S.E.M of data obtained from three independent experiments that yielded similar results. “*” indicates statistically significant () difference of in nicotine-exposed macrophages compared with control and “#” indicates statistically significant () difference in Ae-Og and ascorbic acid supplemented macrophages compared with nicotine-treated macrophages.
